Product Name	NPHS2
Chinese Name	肾小球裂孔膜蛋白PDCN抗体
Alias	nephrosis 2, idiopathic, steroid resistant; NPHS2 gene; PDCN; Podocin; SRN1; PODO_HUMAN.
literatures	Specific References  (6)     |     SL6597R has been referenced in 6 publications. [IF=9.618] Seonhwa Lee. et al. Using Electrochemical Immunoassay in a Novel Microtiter Plate to Detect Surface Markers of Preeclampsia on Urinary Extracellular Vesicles. ACS SENSORS. 2022;XXXX(XXX):XXX-XXX  IA ;  Human.   PubMed:36548998 [IF=6.107] Nan Zang. et al. cGAS-STING Activation by Cytosolic Mitochondrial DNA Contributes to Podocyte Injury in Diabetic Kidney Disease. ISCIENCE. 2022 Sep;:105145  WB ;  Mouse.   PubMed:36176590 [IF=4.72] Zhang, Xiao-Liang, et al. "Vitamin D Prevents Podocyte Injury Via Regulation Of Macrophage M1/M2 Phenotype In Diabetic Nephropathy Rats." Endocrinology (2014).  WB ;  Rat.   PubMed:25188527 [IF=3.414] Cao S et al. Effect of shenyan xiaobai granule on nephrin and podocin of adriamycin-induced renal injury: A randomised controlled trial. J Ethnopharmacol. 2019 Nov 15;244:112104.  IHC-P ;  Rat.   PubMed:31394178 [IF=0] Garovic, Vesna D., and Muthuvel Jayachandran. "DETECTING PODOCYTE INJURY IN DIABETIC NEPHROPATHY AND GLOMERULONEPHRITIS." U.S. Patent No. 20,170,003,299. 5 Jan. 2017.  Human.   PubMed:U.S.PatentNo.20,170,003,299.5 [IF=0] Mohani Chandra Irwanadi. et al. DLBS3233 enhances nephrin and podocin expression also reduces oxidative stress marker and insulin receptor serine diabetic rats’ podocytes. ANN MED SURG. 2023 Mar;:10.1097/MS9.0000000000000308  IHC ;  Rat.   PubMed:10.1097/MS9.0000000000000308
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NPHS2/Podocin: 231-330/383 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Subunit: Interacts with nephrin/NPHS1 and KIRREL. Interacts directly with CD2AP. Interacts with DDN (By similarity). Subcellular Location: Cell membrane; Peripheral membrane protein. Tissue Specificity: Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. DISEASE: Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Similarity: Belongs to the band 7/mec-2 family. SWISS: Q9NP85 Gene ID: 7827 Database links: Entrez Gene: 7827 Human Entrez Gene: 170484 Mouse Entrez Gene: 170672 Rat Omim: 604766 Human SwissProt: Q9NP85 Human SwissProt: Q91X05 Mouse SwissProt: Q8K4G9 Rat Unigene: 412710 Human Unigene: 289099 Mouse Unigene: 86433 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPHS2) Polyclonal Antibody, Unconjugated (SL6597R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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