Product Name	BCAT2
Chinese Name	支链氨基酸转氨酶2抗体
Alias	BCAM; BCAT 2; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; ECA40; Placental protein 18; PP18.
literatures	Specific References  (3)     |     SL6589R has been referenced in 3 publications. [IF=4.079] Jason S. Hinkle. et al. AICAR stimulates mitochondrial biogenesis and BCAA catabolic enzyme expression in C2C12 myotubes. Biochimie. 2021 Nov;:  WB ;  Mouse.   PubMed:34798200 [IF=2.795] Madison E Rivera et al. Effect of valine on myotube insulin sensitivity and metabolism with and without insulin resistance. Mol Cell Biochem . 2020 May;468(1-2):169-183.  WB ;  mouse.   PubMed:32222880 [IF=1.88] Madison E. Rivera. et al. Comparing the effects of palmitate, insulin, and palmitate-insulin co-treatment on myotube metabolism and insulin resistance. 2021 Aug 12  WB ;  Mouse.   PubMed:34382222
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BCAT2: 101-200/392
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Class-IV pyridoxal-phosphate-dependent aminotransferase family members ECA39 and BCAT2 are both enzymes that catalyze the first reaction in the catabolism of the essential branched chain amino acids valine, leucine and isoleucine. ECA39, also known as BCAT1 (branched-chain-amino-acid aminotransferase 1, cytosolic) is localized to the cytoplasm where it forms a homodimer. ECA39 is expressed in the brain and kidney during embryogenesis and is overexpressed in c-Myc induced tumors. BCAT2 (branched-chain-amino-acid aminotransferase 2, mitochondrial), also known as placental protein 18 (PP18), is expressed as two isoforms produced by alternative splicing. The first isoform of BCAT2, designated BCAT2A, is expressed in the mitochondrion, while the second isoform, designated BCAT2B, is expressed in the cytoplasm. Ubiquitously expressed, BCAT2 is also thought to act as a transporter of branched chain α-keto acids. Function: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Subunit: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Subcellular Location: Cytoplasm and Mitochondrion. Tissue Specificity: Ubiquitous. Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. SWISS: O15382 Gene ID: 587 Database links: Entrez Gene: 587 Human Entrez Gene: 12036 Mouse Omim: 113530 Human SwissProt: O15382 Human SwissProt: O35855 Mouse Unigene: 512670 Human Unigene: 24210 Mouse
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-BCAT2 (SL6589R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD Sample: Kidney (Mouse) Tissue Lysate at 30 ug Primary: Anti-BCAT2(SL6589R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BCAT2/BCAM Polyclonal Antibody, Unconjugated(SL6589R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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