Product Name	RNASE4
Chinese Name	核糖核酸酶4抗体
Alias	RAB1; Ribonuclease 4; Ribonuclease A B1; Ribonuclease, RNase A family, 4; RNAS4_HUMAN; RNase 4; RNS4.
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction  vascular endothelial cell  Cytoskeleton  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	14kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RNASE4: 51-147/147
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]. Function: This RNase has marked specificity towards the 3' side of uridine nucleotides. Subcellular Location: Secreted. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P34096 Gene ID: 6038 Database links: Entrez Gene: 6038 Human Entrez Gene: 58809 Mouse Entrez Gene: 56759 Rat SwissProt: P34096 Human SwissProt: Q9JJH1 Mouse SwissProt: O55004 Rat
Product Picture	Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HUVEC cell lysates Primary: Anti-RNASE4 (SL6583R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 15 kDa Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated(SL6583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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