Product Name	NOL8
Chinese Name	核仁蛋白8抗体
Alias	C9orf34; NOL8; NOL8_HUMAN; NOP132; Nucleolar protein 8; Nucleolar protein Nop132.
Research Area	Tumour  TumourCell biologyMaker  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	132kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NOL8: 801-900/1167
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Summary: NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth Function: Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells. Subunit: Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent. Subcellular Location: Nucleus, nucleolus. Note=Localizes in the nucleolar-organizing region during ribosome biogenesis. Tissue Specificity: Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle. Post-translational modifications: Phosphorylated Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q76FK4 Gene ID: 55035 Database links: Entrez Gene: 55035 Human Omim: 611534 Human SwissProt: Q76FK4 Human Unigene: 442199 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NOL8) Polyclonal Antibody, Unconjugated (SL6554R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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