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Product Name ABH8 Chinese Name AlkB同源蛋白8抗体 Alias ABH8; alkB alkylation repair homolog 8 (E. coli); alkB alkylation repair homolog 8; AlkB homologue 8; ALKB8_HUMAN; ALKBH8; Alkylated DNA repair protein alkB homolog 8; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8. Research Area Tumour TumourCell biologyMaker Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 75kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ABH8/ALKBH8: 245-350/664 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Function:
Catalyzes the methylation of 5-carboxymethyl uridine to5-methylcarboxymethyl uridine at the wobble position of theanticodon loop in tRNA. Catalyzes the last step in the formation of5-methylcarboxymethyl uridine at the wobble position of theanticodon loop in target tRNA. Has a preference for tRNA(Arg) andtRNA(Glu), and does not bind tRNA(Lys). Required for normalsurvival after DNA damage. May inhibit apoptosis and promote cellsurvival and angiogenesis.
Subunit:
Interacts with TRMT112.
Subcellular Location:
Cytoplasm. Nucleus. Note=Predominantlycytoplasmic.
Tissue Specificity:
Widely expressed, with highest expression inspleen, followed by pancreas and lung.
Similarity:
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
Q96BT7
Gene ID:
91801
Database links:Entrez Gene: 91801 Human
Entrez Gene: 67667 Mouse
Omim: 613306 Human
SwissProt: Q96BT7 Human
SwissProt: Q80Y20 Mouse
Unigene: 503763 Human
Unigene: 116968 Mouse
Unigene: 440994 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABH8) Polyclonal Antibody, Unconjugated (SL6510R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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