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Product Name SGK3 Chinese Name 丝氨酸/苏氨酸蛋白激酶Sgk3抗体 Alias CISK; Cytokine independent survival kinase; Serine/threonine protein kinase Sgk3; Serine/threonine-protein kinase Sgk3; Serum/glucocorticoid regulated kinase 3; Serum/glucocorticoid regulated kinase family member 3; Serum/glucocorticoid regulated kinase like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK 2; SGK 3; SGK2; Sgk3; SGK3_HUMAN; SGKL. literatures Specific References (5) | SL6475R has been referenced in 5 publications.[IF=5.5] Zhang et al. Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling. (2016) Cell.Physiol.Biochem. 39:360-70 IHC ; Human.[IF=4.858] Qianyu Huo. et al. Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway. Int J Biol Sci. 2021; 17(2): 574–588 WB ; Mouse.[IF=4.65] Zhang, H., et al. "Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling."Cellular Physiology and Biochemistry 39.1 (2016): 360-370. WB ; Human.[IF=3.699] Zijian Ye . et al. MiR-92b-3p inhibits proliferation and migration of C2C12 cells. Cell Cycle. 2020;19(21):2906-2917 WB ; Mouse.[IF=3.251] Yanping Jian. et al. Upregulation of Spinal miR-155-5p Contributes to Mechanical Hyperalgesia by Promoting Inflammatory Activation of Microglia in Bone Cancer Pain Rats. LIFE-BASEL. 2022 Sep;12(9):1349 IF ; Rat.Research Area Tumour Cell biology immunology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SGK3: 51-150/496 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Serine/threonine-protein kinase Sgk3 (SGK3), also designated serum/glucocorticoid regulated kinase 3, belongs to the Ser/Thr protein kinase family of proteins. The serum- and glucocorticoid-regulated kinase proteins are closely related to the Akt protein family. SGK1, a homolog of SGK3, activates ion channels, in particular potassium (K+) channels. SGK2 and SGK3 have been found to also be involved in this activation process, making all three of these proteins important regulators for cell proliferation, epithelial transport and neuromuscular excitability. SGK3 acts as a mediator of IL-3 dependent survival signals in the cell. It localizes to the early endosome and in vesicle-like structures. SGK3 is a widely expressed protein, but it is primarily detected in kidney, liver, pancreas, brain and heart. Phosphorylation of SGK3 at residue Ser 486 leads to an increase in SGK3 activation.
Function:
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Subunit:
Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.
Subcellular Location:
Cytoplasmic vesicle. Early endosome. Localized in vesicle-like structures and in the early endosome.
Tissue Specificity:
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle.
Post-translational modifications:
Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 PX (phox homology) domain.
SWISS:
Q96BR1
Gene ID:
100533105
Database links:Entrez Gene: 100533105 Human
Entrez Gene: 23678 Human
Omim: 607591 Human
SwissProt: Q96BR1 Human
Unigene: 613417 Human
Product Picture Sample:
Lane 1: Mouse Pancreas tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Lane 5: Rat Pancreas tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Breast tissue lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human THP-1 cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-SGK3 (SL6475R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
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