Product Name	phospho-Girdin (Ser1417)
Chinese Name	磷酸化肌动蛋白Binding proteinGirdin抗体
Alias	Girdin (phospho S1417); p-APE(Ser1417); GRDN_HUMAN; CCDC88A; APE; GRDN; KIAA1212; Akt phosphorylation enhancer (APE); Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein (GIV); Girders of actin filament; Hook-related protein 1 (HkRP1);
literatures	Specific References  (1)     |     SL6465R has been referenced in 1 publications. [IF=3.329] Pan S et al. MiR-328-3p inhibits cell proliferation and metastasis in colorectal cancer by targeting Girdin and inhibiting the PI3K/Akt signaling pathway. Exp Cell Res. 2020 May 1;390(1):111939.  WB ;  human.   PubMed:32142853
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Neurobiology  Cell adhesion molecule  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	206kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human APE around the phosphorylation site of Ser1417: QK(p-S)LT
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Function: Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Subunit: Interacts (via C-terminus) with DISC1; the interaction is direct. Interacts with AKT proteins; the interaction is inhibited in presence of DISC1. Homodimer. The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI(4)P] and weakly with phosphatidylinositol 3-phosphate [PI(3)P]. Interacts with microtubules. Interacts with actin through its C-terminal domain. Interacts with the C-terminus of AKT1/PKB. Subcellular Location: Membrane. Cell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle. Cell projection, lamellipodium. Note=Localizes to the cell membrane through interaction with phosphoinositides. Tissue Specificity: Expressed ubiquitously. Post-translational modifications: Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. Similarity: Belongs to the CCDC88 family. SWISS: Q3V6T2 Gene ID: 55704 Database links: Entrez Gene: 55704 Human Entrez Gene: 108686 Mouse Entrez Gene: 305605 Rat SwissProt: Q3V6T2 Human SwissProt: Q5SNZ0 Mouse
Product Picture	Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-phospho-APE(Ser1417) (SL6465R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 206 kD Observed band size: 206 kD Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-APE(Ser1417) antibody (SL6465R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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