Product Name	EGR3
Chinese Name	早期生长反应蛋白3抗体
Alias	early growth response 3; early growth response gene 3; early growth response protein 3; early growth responsive 3; EGR-3; MGC138484; pilot; zinc finger protein pilot; EGR3_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EGR3: 201-300/387
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	EGR3 is a member of the early growth response transcription factor family of C2H2 zinc finger proteins (other members EGR1, EGR2 and EGR4). EGR proteins are immediate early proteins, expression of which is swiftly upregulated in response to a wide range of extracellular stimuli. EGR3 is also thought to be involved in development of muscle spindles, and is upregulated in several regions of the brain in response to stress or injury. Function: Probable transcription factor involved in muscle spindle development. Subcellular Location: Nuclear. Similarity: Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: Q06889 Gene ID: 1960 Database links: Entrez Gene: 1960 Human Entrez Gene: 13655 Mouse Entrez Gene: 25148 Rat Omim: 602419 Human SwissProt: Q06889 Human SwissProt: P43300 Mouse SwissProt: P43301 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EGR3) Polyclonal Antibody, Unconjugated (SL6448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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