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Product Name PRLR Chinese Name 泌乳素受体抗体 Alias Lactogen receptor; MGC105486; OPR; Pr-1; Pr-3; PRL R; PRL-R; PRLR; Prlr-rs1; PRLR_HUMAN; Prolactin receptor a; Prolactin receptor; Prolactin receptor delta 7/11; RATPRLR; Secreted prolactin binding protein; Truncated testis-specific box 1-C prolactin receptor; wu:fj65c07; AI987712; CLONE SPM213; CPRLP; Delta 4-delta 7/11 truncated prolactin receptor; Delta 4-SF1b truncated prolactin receptor; hPRL receptor; hPRLrI. literatures Specific References (4) | SL6445R has been referenced in 4 publications.[IF=4.879] Senta Georgia. et al. Exogenous Lactogenic Signaling Stimulates Beta Cell Replication In Vivo and In Vitro. Biomolecules. 2022 Feb;12(2):215 IHC ; Mouse.[IF=3.263] Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190. WB ; dairy cow.[IF=2.425] Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1). IHC-P ; muskrat.[IF=2.005] Yongfu La. et al. Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak. 2021 Nov 09 WB ; Yak.Research Area Tumour Cell biology immunology Signal transduction Growth factors and hormones Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 67kDa Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Prolactin Receptor: 251-350/622 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011].
Function:
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Subunit:
Homodimer upon hormone binding. Interacts with SMARCA1. Interacts with GH1. Interacts with CSH. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Isoform 7: Secreted.
Tissue Specificity:
Expressed in breast, placenta, kidney, liver and pancreas.
Similarity:
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
SWISS:
P16471
Gene ID:
5618
Database links:Entrez Gene: 100053793 Horse
Entrez Gene: 5618 Human
Omim: 176761 Human
SwissProt: P16471 Human
SwissProt: P14787 Rabbit
SwissProt: O46561 Sheep
Unigene: 368587 Human
Unigene: 9757 Rat
Product Picture Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-PRLR (SL6445R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRLR Polyclonal Antibody, Unconjugated(SL6445R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRLR) Polyclonal Antibody, Unconjugated (SL6445R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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