Product Name	UHRF1
Chinese Name	核蛋白95抗体
Alias	UHRF1_HUMAN; E3 ubiquitin-protein ligase UHRF1; ICBP90; NP95; RNF106; TDRD22; hUHRF1; huNp95; EC:2.3.2.27; Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95 (HuNp95; hNp95); RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; ubiquitin like with PHD and ring finger domains 1;
literatures	Specific References  (1)     |     SL6427R has been referenced in 1 publications. [IF=1.12] Babacan, Nalan A., et al. "The prognostic value of UHRF-1 and p53 in gastric cancer." Saudi Journal of Gastroenterology 22.1 (2016): 25.  IHC-P ;  Human.   PubMed:26831603
Research Area	Tumour  Cell biology  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Horse, Guinea Pig, )
Applications	ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	90kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human UHRF1: 701-793/793
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014] Function: Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. Subunit: Interacts with histones H3, H1 and H2B (By similarity). Interacts with HDAC1, but not with HDAC2. Interacts with UHRF1BP1. Binds methylated CpG containing oligonucleotides. Subcellular Location: Nucleus. Tissue Specificity: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. Post-translational modifications: Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity. Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. Similarity: Contains 1 PHD-type zinc finger. Contains 2 RING-type zinc fingers. Contains 1 ubiquitin-like domain. Contains 1 YDG domain. SWISS: Q96T88 Gene ID: 29128 Database links: Entrez Gene: 29128 Human Omim: 607990 Human SwissProt: Q96T88 Human Unigene: 108106 Human UHRF1是一种核蛋白，通过DNA解螺旋酶而识别甲基化位点，UHRF1与一些组蛋白的结合事件对于维持正确的Epigenetics控制具有非常重要的意义。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICBP90) Polyclonal Antibody, Unconjugated (SL6427R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-ICBP90 antibody (SL6427R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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