Product Name	Caspase-1 p20
Chinese Name	天冬氨酸-胱氨酸特异性蛋白酶家族抗体
Alias	caspase-1 isoform alpha p20 subunit; CASP 1; CASP1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BC; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p20; CASP1_HUMAN.
literatures	Specific References  (5)     |     SL6368R has been referenced in 5 publications. [IF=8.039] Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:37078976 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020.Dec;10(8):e240.  WB ;  Mouse.   PubMed:33377656 [IF=4.858] Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163–3173  WB ;  Rat, Human.   PubMed:33162822 [IF=3.361] Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.  IHC,WB ;  rat.   PubMed:32193099 [IF=3.06] Zhang, Bo, et al. "Cortistatin inhibits NLRP3 inflammasome activation of cardiac fibroblasts during sepsis." Journal of Cardiac Failure (2015).  WB ;  Rat.   PubMed:25639691
Research Area	Tumour  Cell biology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20/46kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Caspase-1 p20: 188-290/404
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]. Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Post-translational modifications: The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P29466 Gene ID: 834 Database links: Entrez Gene: 834 Human Entrez Gene: 12362 Mouse Entrez Gene: 25166 Rat Omim: 147678 Human SwissProt: P29466 Human SwissProt: P29452 Mouse SwissProt: P43527 Rat Unigene: 2490 Human Unigene: 1051 Mouse Unigene: 37508 Rat
Product Picture	Sample: Lane 1: Mouse Thymus tissue lysates Lane 2: Rat Thymus tissue lysates Lane 3: Human THP-1 cell lysates Lane 4: Human U937 cell lysates Primary: Anti-Caspase-1 p20 (SL6368R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46 kDa Observed band size: 50 kDa Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated(SL6368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (SL6368R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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