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Product Name Glutamine PRPP amidotransferase Chinese Name 谷氨酰胺磷酸核糖基焦磷酸酰胺基转移酶 Alias Amidophosphoribosyltransferase [Precursor]; ATASE; Glutamine phosphoribosylpyrophosphate amidotransferase; Glutamine PRPP amidotransferase; GPAT; Phosphoribosyl pyrophosphate amidotransferase; PPAT; PRAT; PUR1_HUMAN. literatures Specific References (1) | SL6359R has been referenced in 1 publications.[IF=6.59] Pengfei Zhang. et al. Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia. FRONT NUTR. 2022 Dec 19;9:1045805 WB ; Mouse.Research Area Cell biology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 56kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Glutamine PRPP amidotransferase: 351-430/517 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Phosphoribosyl pyrophosphate amidotransferase (PPAT) is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. The genes for PPAT and PAICS/AIRC, (a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway), are located in close proximity on chromosome 4.
Subunit:
Homotetramer.
Tissue Specificity:
Ubiquitously expressed.
Similarity:
n the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Contains 1 glutamine amidotransferase type-2 domain.
SWISS:
Q06203
Gene ID:
5471
Database links:Entrez Gene: 5471 Human
Entrez Gene: 231327 Mouse
Omim: 172450 Human
SwissProt: Q06203 Human
Unigene: 331420 Human
Unigene: 202337 Mouse
Unigene: 18690 Rat
Product Picture Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamine PRPP amidotransferase Polyclonal Antibody, Unconjugated(SL6359R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamine PRPP amidotransferase Polyclonal Antibody, Unconjugated(SL6359R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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