TEL: +86 571 56623320 EMAIL: [email protected]
Product Name DAP1 Chinese Name 死亡相关蛋白1抗体 Alias DAP 1; DAP; DAP-1; DAP1_HUMAN; Death associated protein 1; Death associated protein; Death-associated protein 1. Research Area Cell biology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 11kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DAP1: 61-102/102 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail his gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma.
Function:
Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.
Post-translational modifications:
Phosphorylated. Phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy.
SWISS:
P51397
Gene ID:
1611
Database links:Entrez Gene: 1611 Human
Entrez Gene: 223453 Mouse
Omim: 600954 Human
SwissProt: P51397 Human
SwissProt: Q91XC8 Mouse
Unigene: 75189 Human
Unigene: 222867 Mouse
Unigene: 1531 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAP1) Polyclonal Antibody, Unconjugated (SL6351R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAP1) Polyclonal Antibody, Unconjugated (SL6351R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAP1) Polyclonal Antibody, Unconjugated (SL6351R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAP1) Polyclonal Antibody, Unconjugated (SL6351R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode