Product Name	VGF
Chinese Name	神经生长因子诱导蛋白抗体
Alias	NERP-1; NERP-2; Nerve growth factor inducible; Neuroendocrine regulatory peptide-2; Neurosecretory protein VGF; VGF_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Cow, Horse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	cytoplasmic Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human VGF: 451-550/615
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity Function: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. Subcellular Location: Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. Tissue Specificity: Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. Post-translational modifications: Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure. SWISS: O15240 Gene ID: 7425 Database links: Entrez Gene: 7425 Human Entrez Gene: 381677 Mouse Entrez Gene: 29461 Rat Omim: 602186 Human SwissProt: O15240 Human SwissProt: P20156 Rat Unigene: 587325 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VGF) Polyclonal Antibody, Unconjugated (SL6349R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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