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Product Name Caveolin-2 Chinese Name 细胞质膜微囊蛋白-2抗体 Alias CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294. Research Area Cardiovascular Cell biology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Caveolin-2: 41-140/162 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Function:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Subunit:
Monomer or homodimer.
Subcellular Location:
Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein.
Tissue Specificity:
Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
Post-translational modifications:
Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
Similarity:
Belongs to the caveolin family.
SWISS:
P51636
Gene ID:
858
Database links:Entrez Gene: 858 Human
Entrez Gene: 12390 Mouse
Omim: 601048 Human
SwissProt: P51636 Human
SwissProt: Q9WVC3 Mouse
Unigene: 212332 Human
Unigene: 603096 Human
Unigene: 396075 Mouse
Unigene: 81070 Rat
Caveolin是细胞生长相关信号途径及Tumour发生发展过程中重要的抑制因子,Caveolae是The cell membrane内的特殊膜结构,参与包括细胞的分子运输、细胞粘附和Signal transduction在内的多种细胞活动。Caveolin-1是Caveolae中重要的结构蛋白,抑制细胞生长,与多种人类Tumour发生发展相关的信号分子相互作用。Caveolin在Signal transduction的整合中起支架蛋白的作用。Caveolin构成了一个蛋白家族,他们是细胞质膜中发夹样结构域的主要结构成分。Caveolin在Signal transduction的整合中起支架蛋白的作用。至今已经鉴定了3种Caveolin (Caveolin-1、2 和3),它们具有不同的组织分布。Product Picture Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caveolin-2) Polyclonal Antibody, Unconjugated (SL6310R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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