Product Name	Acid Phosphatase
Chinese Name	酸性磷酸酶抗体
Alias	Acid phosphatase 1 soluble; Acid phosphatase of erythrocyte; Adipocyte acid phosphatase; Cytoplasmic phosphotyrosyl protein phosphatase; HAAP; Low molecular weight phosphotyrosine protein phosphatase; PAP1; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1; PPAC_HUMAN.
Research Area	Tumour  Cell biology  Cyclin  Kinases and Phosphatases  TumourCell biologyMaker
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Acid Phosphatase: 71-158/158
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Phosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols, e.g. a hexose phosphate, to yield the alcohol and free inorganic phosphate. This may be a way of recycling phosphate in the cell, and the level of phosphate may be elevated under conditions of phosphate starvation (in algae for example). Acid phosphatases have pH optima below 7, whereas alkaline phosphatases are most active above pH 7. Commercial quantities of acid phosphatase are obtained from plant sources e.g. potato and wheat germ, whereas similar quantities of alkaline phosphatase are obtained from animal intestine and bacteria. Function: Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. Subunit: Isoform 1 interacts with the SH3 domain of SPTAN1. There is no interaction observed for isoforms 2 or 3. Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1. Subcellular Location: Cytoplasm. Tissue Specificity: T-lymphocytes express only isoform 2. Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family. SWISS: P24666 Gene ID: 52 Database links: Entrez Gene: 52 Human Entrez Gene: 11431 Mouse Entrez Gene: 631286 Mouse Entrez Gene: 24161 Rat Omim: 171500 Human SwissProt: P24666 Human SwissProt: Q9D358 Mouse SwissProt: P41498 Rat Unigene: 558296 Human Unigene: 359831 Mouse Unigene: 108187 Rat Unigene: 202830 Rat Unigene: 220315 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Acid Phosphatase) Polyclonal Antibody, Unconjugated (SL6291R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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