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Product Name UEVLD Chinese Name Ubiquitin结合酶E2样蛋白抗体 Alias ATTP; Ubiquitin-conjugating enzyme E2 variant 3; EV and lactate malate dehydrogenase domain containing protein; EV and lactate/malate dehydrogenase domain-containing protein; signaling molecule ATTP; ubiquitin conjugating enzyme E2 like; Ubiquitin conjugating enzyme E2 variant 3; Ubiquitin E2 variant and lactate/malate dehydrogenase domain containing protein; Ubiquitin-conjugating enzyme E2 variant 3; UEV 3; UEV and lactate malate dehyrogenase domains; UEV-3; UEV2 and LDH domains containing protein; UEV3; uevld; UEVLD_HUMAN. Research Area Tumour Cell biology Signal transduction Cyclin TumourCell biologyMaker Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 52kDa Cellular localization Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human UEVLD: 85-180/471 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail UEV3, also known as EV and lactate/malate dehydrogenase domain-containing protein, is a 471 amino acid protein that contains one UEV (ubiquitin E2 variant) domain, which typically interacts with ubiquitin. UEV3 is thought to be a paralogue of tsg 101, a protein that exerts regulatory effects on E2 activity in cellular ubiquitination processes. With amino-terminal homology to the catalytic domain of ubiquitin-conjugating enzymes, it is thought that UEV3 may function as a negative regulator of polyubiquitination. UEV3 is expressed in various colon carcinoma cell lines, carcinomas of the uterine cervix and peripheral blood leukocytes as well as normal colon and cervical epithelium.
Subunit:
Homodimer.
Tissue Specificity:
Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
Similarity:
In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
In the C-terminal section; belongs to the LDH/MDH superfamily.
Contains 1 UEV (ubiquitin E2 variant) domain.
SWISS:
Q8IX04
Gene ID:
55293
Database links:Entrez Gene: 55293 Human
Omim: 610985 Human
SwissProt: Q8IX04 Human
Unigene: 407991 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UEVLD) Polyclonal Antibody, Unconjugated (SL6287R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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