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Product Name GNAQ Chinese Name G蛋白α亚单位蛋白Q抗体 Alias G ALPHA q; G protein alpha Q; GAQ; Guanine nucleotide binding protein alpha q; Guanine nucleotide binding protein G q subunit alpha; GNAQ_HUMAN. Research Area Tumour Cardiovascular Cell biology Neurobiology Signal transduction G protein-coupled receptor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization The nucleus The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GNAQ: 51-150/359 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GNAQ belong to the family of heterotrimeric Guanine nucleotide-binding proteins proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. GNAQ is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta. in recent studies over expression of GNAQ is associated with cardiomyocyte apoptosis.
Function:
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity).
Subunit:
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Binds SLC9A3R1. Forms a complex with PECAM1 and BDKRB2. Interacts with PECAM1.
Subcellular Location:
Nucleus (By similarity). Membrane (By similarity). Nucleus membrane (By similarity). Note=Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes (By similarity).
Tissue Specificity:
Predominantly expressed in ovary, prostate, testis and colon.
Similarity:
Belongs to the G-alpha family. G(q) subfamily.
SWISS:
P50148
Gene ID:
2776
Database links:Entrez Gene: 2776 Human
Entrez Gene: 14682 Mouse
Omim: 600998 Human
SwissProt: P50148 Human
SwissProt: P21279 Mouse
Unigene: 269782 Human
Unigene: 439701 Mouse
Unigene: 441601 Mouse
Unigene: 22817 Rat
Gαq是G蛋白家族成员之一,是Gq蛋白的功能单位。其功能为传导来自GPCR的信号到细胞内。研究证明,Gαq与免疫细胞活化、增殖、死亡、分化、迁移,cell factor分泌以及自身免疫等均有非常密切的关系。Product Picture Sample:
SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti-GNAQ (SL6152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-GNAQ (SL6152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti-GNAQ (SL6152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GNAQ/Gα q Polyclonal Antibody, Unconjugated(SL6152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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