Product Name	Frizzled 1/Wnt receptor
Chinese Name	Wnt信号受体蛋白/Frizzled homolog 1抗体
Alias	Frizzled-1; Frizzled homolog 1 (Drosophila); Fz1; hFz1; Fz-1; FZD1; FzE1; Wnt receptor; FZD1_HUMAN.
Research Area	Tumour  Cell biology  immunology  Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=3ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	63/71kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Frizzled 1: 501-600/647
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK3 kinase, nuclear accumulation of beta catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt mediated inactivation of GSK3 kinase. Both pathways seem to involve interactions with G proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Subcellular Location: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (By similarity). Tissue Specificity: Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. Similarity: Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain. SWISS: Q9UP38 Gene ID: 8321 Database links: Entrez Gene: 445417 Cow Entrez Gene: 8321 Human Entrez Gene: 14362 Mouse Entrez Gene: 58868 Rat Omim: 603408 Human SwissProt: Q9UP38 Human SwissProt: O70421 Mouse SwissProt: Q08463 Rat Unigene: 94234 Human Unigene: 246003 Mouse Unigene: 6575 Rat
Product Picture	Blank control: A549. Primary Antibody (green line): Rabbit Anti-Frizzled 1 antibody (SL6148R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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