Product Name	Wnt8A
Chinese Name	信号通路WNT8A抗体
Alias	Protein Wnt-8a; Protein Wnt-8d; wingless type MMTV integration site family, member 8A; WNT 8A; Wnt 8d; wnt8a; WNT8A_HUMAN; WNT8D.
literatures	Specific References  (1)     |     SL6129R has been referenced in 1 publications. [IF=6.208] Dong Zhang. et al. Exosomes Derived from Adipose Stem Cells Enhance Bone Fracture Healing via the Activation of the Wnt3a/β-Catenin Signaling Pathway in Rats with Type 2 Diabetes Mellitus. INT J MOL SCI. 2023 Jan;24(5):4852  WB ;  Rat.   PubMed:36902283
Research Area	Tumour  Cell biology  immunology  Chromatin and nuclear signals  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Wnt8A: 201-300/351
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein. Function: Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus. Subcellular Location: Secreted, extracellular space, extracellular matrix. Similarity: Belongs to the Wnt family. SWISS: Q9H1J5 Gene ID: 7478 Database links: Entrez Gene: 7478 Human Omim: 606360 Human SwissProt: Q9H1J5 Human Unigene: 591274 Human
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Wnt8A (SL6129R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 48 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt8A Polyclonal Antibody, Unconjugated(SL6129R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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