Product Name	ZNF185
Chinese Name	Zinc finger protein185抗体
Alias	LIM domain protein ZNF185; P1 A; Zinc finger protein 185 (LIM domain); ZN185_HUMAN.
Research Area	Tumour  Chromatin and nuclear signals  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=3ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	76(human)/39(mo,kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ZNF185: 551-650/689
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation. Function: May be involved in the regulation of cellular proliferation and/or differentiation. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Tissue Specificity: Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood. Similarity: Contains 1 LIM zinc-binding domain. SWISS: O15231 Gene ID: 7739 Database links: Entrez Gene: 7739 Human Entrez Gene: 22673 Mouse Omim: 300381 Human SwissProt: O15231 Human SwissProt: Q62394 Mouse Unigene: 16622 Human Unigene: 1161 Mouse
Product Picture	Sample: MCF-7(Human) Cell Lysate at 40 ug A431(Human) Cell Lysate at 40 ug U2os(Human) Cell Lysate at 40 ug Primary: Anti-ZNF185 (SL6119R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76/39 kD Observed band size: 67 kD Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (SL6199R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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