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Product Name SPARCL1 Chinese Name Extracellular matrix蛋白2抗体 Alias Ecm2; SPRL1; RAGS1 antigen; Extracellular matrix protein 2; Hevin; High endothelial venule protein; MAST 9; mast9; Matrix glycoprotein Sc1; PIG33; proliferation inducing protein 33; SC1; SPARC like 1; SPARC-like protein 1; SPRL1_HUMAN. literatures Specific References (3) | SL6110R has been referenced in 3 publications.[IF=5.959] Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852. IHF&WB ; Mouse.[IF=3.9] Wallingford et al. Altered Developmental Expression of the Astrocyte-Secreted Factors Hevin and SPARC in the Fragile X Mouse Model. (2017) Front.Mol.Neurosci. 10:268 ICC,WB ; Mouse.[IF=0] "Wallingford, J. (2016). Astrocyte-Secreted Factors in Fragile X Mice (Doctoral dissertation).Diss. WB, IF(ICC) ; Mouse.Research Area Tumour Zinc finger protein Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 74kDa Cellular localization Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SPARCL1/Ecm2: 601-664/664 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1.
Subcellular Location:
Secreted, extracellular space, extracellular matrix.
Tissue Specificity:
Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Post-translational modifications:
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
Similarity:
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
SWISS:
Q14515
Gene ID:
8404
Database links:Entrez Gene: 8404 Human
Omim: 606041 Human
SwissProt: Q14515 Human
Unigene: 62886 Human
Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SPARCL1 (SL6110R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kD
Observed band size: 73 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (SL6110R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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