Product Name	SIRT3
Chinese Name	Mitochondrion乙酰化酶3抗体
Alias	hSIRT3; hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIRT 3; SIRT3_HUMAN; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3.
literatures	Specific References  (3)     |     SL6105R has been referenced in 3 publications. [IF=4.546] Su ShiJie. et al. Kai-Xin-San protects against mitochondrial dysfunction in Alzheimer’s disease through SIRT3/NLRP3 pathway. CHIN MED-UK. 2023 Dec;18(1):1-16  WB,IF,ICC ;  Mouse.   PubMed:36918872 [IF=3.448] Liu L et al. Melatonin ameliorates cerebral ischemia/reperfusion injury through SIRT3 activation. Life Sci. 2019 Nov 4;239:117036.  IHC-P ;  Mouse.   PubMed:31697951 [IF=0.496] Chuanjiang He. et al. MiRNA-494 specifically inhibits SIRT3-mediated microglia activation in sepsis-associated encephalopathy. TRANSL CANCER RES. 2022 Jul; 11(7): 2299–2309  WB, IHC ;  Rat.   PubMed:35966295
Research Area	Tumour  Cardiovascular  Cell biology  Cyclin  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SIRT3: 201-300/399
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control. [provided by RefSeq, May 2019] Function: NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. Subunit: Interacts with NDUFA9, ACSS1, IDH2 and GDH. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Widely expressed. Post-translational modifications: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. Similarity: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. SWISS: Q9NTG7 Gene ID: 23410 Database links: Entrez Gene: 23410 Human Entrez Gene: 64384 Mouse SwissProt: Q9NTG7 Human SwissProt: Q8R104 Mouse
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-SIRT3 (SL6105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD Sample: Liver (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-SIRT3 (SL6105R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (SL6105R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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