TEL: +86 571 56623320 EMAIL: [email protected]
Product Name SIRT3 Chinese Name Mitochondrion乙酰化酶3抗体 Alias hSIRT3; hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIRT 3; SIRT3_HUMAN; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3. literatures Specific References (3) | SL6105R has been referenced in 3 publications.[IF=4.546] Su ShiJie. et al. Kai-Xin-San protects against mitochondrial dysfunction in Alzheimer’s disease through SIRT3/NLRP3 pathway. CHIN MED-UK. 2023 Dec;18(1):1-16 WB,IF,ICC ; Mouse.[IF=3.448] Liu L et al. Melatonin ameliorates cerebral ischemia/reperfusion injury through SIRT3 activation. Life Sci. 2019 Nov 4;239:117036. IHC-P ; Mouse.[IF=0.496] Chuanjiang He. et al. MiRNA-494 specifically inhibits SIRT3-mediated microglia activation in sepsis-associated encephalopathy. TRANSL CANCER RES. 2022 Jul; 11(7): 2299–2309 WB, IHC ; Rat.Research Area Tumour Cardiovascular Cell biology Cyclin Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 43kDa Cellular localization cytoplasmic Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SIRT3: 201-300/399 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control. [provided by RefSeq, May 2019]
Function:
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Subunit:
Interacts with NDUFA9, ACSS1, IDH2 and GDH.
Subcellular Location:
Mitochondrion matrix.
Tissue Specificity:
Widely expressed.
Post-translational modifications:
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Similarity:
Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
SWISS:
Q9NTG7
Gene ID:
23410
Database links:Entrez Gene: 23410 Human
Entrez Gene: 64384 Mouse
SwissProt: Q9NTG7 Human
SwissProt: Q8R104 Mouse
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-SIRT3 (SL6105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-SIRT3 (SL6105R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (SL6105R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode
Scan Whatsapp Qrcode