Product Name	RIL
Chinese Name	抑癌基因PDLIM4抗体
Alias	Enigma homolog; LIM domain protein; LIM protein RIL; PDLIM 4; PDLIM4; PDZ and LIM domain 4; PDZ and LIM domain protein 4; Reversion induced LIM protein; PDLI4_HUMAN.
Research Area	Tumour  Chromatin and nuclear signals  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RIL: 251-330/330
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	RIL, also known as PDLIM4 is a putative tumor suppressor gene. RIL is transcriptionally repressed by hypermethylation in a variety of tumors including breast and prostate. The difference in methylation status of RIL between normal and cancerous tissues is being investigated as to determine the use of RIL as a potential biomarker. Two isoforms of human RIL one have been identified. Subcellular Location: Cytoplasmic Similarity: Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain. SWISS: P50479 Gene ID: 8572 Database links: Entrez Gene: 8572 Human Entrez Gene: 30794 Mouse Entrez Gene: 24915 Rat Omim: 603422 Human SwissProt: P50479 Human SwissProt: P70271 Mouse SwissProt: P36202 Rat Unigene: 424312 Human Unigene: 21830 Mouse Unigene: 34221 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIL) Polyclonal Antibody, Unconjugated (SL6093R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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