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Product Name TBP Chinese Name TATABinding protein抗体 Alias TBP_HUMAN; TATA-box-binding protein; TATA-box binding protein; GTF2D1; TF2D; TFIID; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; HDL4; TBP1; GTF2D; SCA17; Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 在DNA结合多蛋白因子TFIID的核心起作用的一般转录因子。TFIID与TATA盒的结合是pre-initiation complex(PIC)的初始转录步骤,在RNA聚合酶II转录的真核基因激活中起作用。也是一种含有BRF2的转录因子复合物的成分,调节RNA聚合酶III介导的转录。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse heart
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 38 kDa
Observed MW: 38 kDa
Tissue: Mouse testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SL60215R
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