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Product Name DAL1/EPB41L3 Chinese Name Cytoskeleton4.1蛋白家族DAL1抗体 Alias Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN. Research Area Tumour Cell biology immunology Neurobiology Cytoskeleton The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 120kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DAL1/EPB41L3: 131-230/1087 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail EPB41L3 is an actin-binding protein originally identified as a molecule whose expression is down-regulated in lung adenocarcinoma. EPB41L3 is a member of the protein 4.1 superfamily, which encompasses structural proteins that play important roles in membrane processes via interactions with actin, spectrin, and the cytoplasmic domains of integral membrane proteins. The gene for EPB41L3 is thought to function as a tumor suppressor and localizes within chromosomal region 18p11.3, which is affected by loss of heterozygosity (LOH) in various adult tumors.
Function:
Critical growth regulator in the pathogenesis of meningiomas.
Subunit:
Interacts with CADM1.
Subcellular Location:
Cytoplasm, cytoskeleton
Tissue Specificity:
Expressed at high levels in brain, with lower levels in kidney, intestine, and testis.
Similarity:
ontains 1 FERM domain.
SWISS:
Q9Y2J2
Gene ID:
23136
Database links:Entrez Gene: 23136 Human
Entrez Gene: 13823 Mouse
Omim: 605331 Human
SwissProt: Q9Y2J2 Human
SwissProt: Q9WV92 Mouse
Unigene: 213394 Human
Unigene: 131135 Mouse
Product Picture Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
U-87MG (Human) CellLysate at 30 ug
HepG2 (Human) CellLysate at 30 ug
Primary: Anti-DAL1/EPB41L3 (SL6003R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 130-140 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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