Product Name	HSP40
Chinese Name	热休克蛋白家族40抗体
Alias	HSP40 homolog; DnaJ (Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1.
Research Area	Tumour  Cell biology  immunology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DNAJB4/HSP40 homolog: 151-250/337
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DNAJB4 is a member of the evolutionarily conserved DNAJ/HSP40 protein family. Function: Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Subunit: Homodimer. Subcellular Location: Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Tissue Specificity: Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver. Similarity: Contains 1 J domain. SWISS: Q9UDY4 Gene ID: 3337 Database links: Entrez Gene: 3337 Human Entrez Gene: 81489 Mouse Entrez Gene: 361384 Rat Omim: 604572 Human SwissProt: P25685 Human SwissProt: Q9QYJ3 Mouse Unigene: 515210 Human Unigene: 282092 Mouse Unigene: 128947 Rat 热休克蛋白家族40抗体
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP40) Polyclonal Antibody, Unconjugated (SL6002R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSP40 Polyclonal Antibody, Unconjugated(SL6002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining This image has been kindly submitted by Tübingen Ageing and Tumour Immunology Group (TATI), Centre for Medical Research (ZMF), University of Tübingen Medical School www.tati-group.de via our distributor Biozol.Human Melanoma cell line, EST02, was stained with Rabbit Anti-HSP40 homolog Polyclonal Antibody, FITC Conjugated (SL6002-FITC) at 1:50 for 30 minutes, on ice.

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