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Product Name RAD54B Chinese Name DNA修复和重组蛋白RAD54B抗体 Alias DNA repair and recombination protein RAD54B; Fibrinogen silencer binding protein; FSBP; RAD54 homolog B; RA54B_HUMAN. Research Area Tumour Cell biology immunology Chromatin and nuclear signals Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 103kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human RAD54B: 821-910/910 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
Function:
Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.
Subunit:
Interacts with RAD51 through the NH2-terminal domain. Immunoprecipitation experiments show that the interaction is constitutive and not induced by ionizing radiation. The interaction may be indirect.
Subcellular Location:
Nucleus (Probable).
Tissue Specificity:
Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.
Similarity:
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
SWISS:
Q9Y620
Gene ID:
25788
Database links:Entrez Gene: 25788 Human
Entrez Gene: 623474 Mouse
Omim: 604289 Human
SwissProt: Q9Y620 Human
SwissProt: Q6PFE3 Mouse
Unigene: 30561 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAD54B) Polyclonal Antibody, Unconjugated (SL5940R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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