Product Name	ADAM12
Chinese Name	去整合素样金属蛋白酶12抗体
Alias	A disintegrin and metalloproteinase domain 12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766.
literatures	Specific References  (1)     |     SL5847R has been referenced in 1 publications. [IF=5.656] Miranda da Costa NM et al. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways. Cells. 2019 Jul 17;8(7). pii: E731.  IHC-P ;  Human.   PubMed:31319505
Research Area	Tumour  Cell biology  immunology  Signal transduction  Cell adhesion molecule  Cytoskeleton  Extracellular matrix  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=3μg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	77/100kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ADAM12: 201-300/909 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). Subunit: Interacts with alpha-actinin-2 and with syndecans (By similarity). Interacts with SH3PXD2A. Interacts with FST3. Interacts with GNB2L1/RACK1; the interaction is required for PKC-dependent translocation of ADAM12 to the cell membrane. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted (Potential). Isoform 4: Secreted (Potential). Tissue Specificity: Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. Post-translational modifications: The precursor is cleaved by a furin endopeptidase (By similarity). Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: O43184 Gene ID: 8038 Database links: Entrez Gene: 8038 Human Entrez Gene: 11489 Mouse Entrez Gene: 679837 Rat Omim: 602714 Human SwissProt: O43184 Human SwissProt: Q61824 Mouse Unigene: 594537 Human Unigene: 439714 Mouse
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ADAM12 (SL5847R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77/100 kD Observed band size: 115 kD Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-ADAM12 antibody (SL5847R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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