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Rabbit Anti-ASAP3 antibody
Rabbit Anti-ASAP3 antibody
ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1; ACAP4; Arf GAP with SH3 domain; Arf GAP with SH3 domain, ANK repeat and PH domain containing protein 3; ARF6 GTPase activating protein; ArfGAP with SH3 domain, ankyrin repeat and PH domain; Centaur
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  • NO.:SL5832R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Sheep,)
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Details

Product Name ASAP3
Chinese Name GTP酶激活蛋白ASAP3抗体
Alias ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1; ACAP4; Arf GAP with SH3 domain; Arf GAP with SH3 domain, ANK repeat and PH domain containing protein 3; ARF6 GTPase activating protein; ArfGAP with SH3 domain, ankyrin repeat and PH domain; Centaurin, beta 6; CENTB6; DDEFL1; Development and differentiation enhancing factor like 1; Up regulated in liver cancer 1 (UPLC1); UPLC1.  
Research Area Tumour  immunology  Signal transduction  Cell adhesion molecule  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 99kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human ASAP3: 251-350/903 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail ASAP3 is a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion.

Function:
Promotes cell proliferation.

Subunit:
Cytoplasm.

Tissue Specificity:
Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes.

Similarity:
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.

SWISS:
Q8TDY4

Gene ID:
55616

Database links:

Entrez Gene: 55616 Human

Entrez Gene: 523797 Cow

SwissProt: Q8TDY4 Human

Unigene: 437379 Human



Product Picture
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- ASAP3 (SL5832R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (SL5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (SL5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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