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Product Name ASAP3 Chinese Name GTP酶激活蛋白ASAP3抗体 Alias ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1; ACAP4; Arf GAP with SH3 domain; Arf GAP with SH3 domain, ANK repeat and PH domain containing protein 3; ARF6 GTPase activating protein; ArfGAP with SH3 domain, ankyrin repeat and PH domain; Centaurin, beta 6; CENTB6; DDEFL1; Development and differentiation enhancing factor like 1; Up regulated in liver cancer 1 (UPLC1); UPLC1. Research Area Tumour immunology Signal transduction Cell adhesion molecule Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 99kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ASAP3: 251-350/903 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ASAP3 is a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion.
Function:
Promotes cell proliferation.
Subunit:
Cytoplasm.
Tissue Specificity:
Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes.
Similarity:
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.
SWISS:
Q8TDY4
Gene ID:
55616
Database links:Entrez Gene: 55616 Human
SwissProt: Q8TDY4 Human
Unigene: 437379 Human
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- ASAP3 (SL5832R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (SL5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (SL5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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