Product Name	PHLP
Chinese Name	光导蛋白样蛋白PDCL抗体
Alias	PDCL; Phosducin Like; PhLP; Phosducin-Like Protein; PHLOP1; Phosducin like protein; PhLP1; PHLP_HUMAN.
Research Area	Cell biology  Signal transduction  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PHLP: 51-150/301
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008] Function: Acts as a positive regulator of hedgehog signaling and regulates ciliary function. Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Isoform 2: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin. Subunit: Forms a complex with the beta and gamma subunits of the GTP-binding protein, transducin. Interacts with the CCT chaperonin complex. Tissue Specificity: Expressed in buccal mucosa cell and 236 other tissues Similarity: Belongs to the phosducin family. SWISS: Q13371 Gene ID: 5082 Database links: Entrez Gene: 5082 Human Entrez Gene: 67466 Mouse Entrez Gene: 64013 Rat Omim: 604421 Human SwissProt: Q13371 Human SwissProt: Q9DBX2 Mouse SwissProt: Q63737 Rat Unigene: 271749 Human Unigene: 620774 Human Unigene: 30709 Mouse Unigene: 51153 Rat
Product Picture	Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Rat Heart tissue lysates Lane 5: Rat Liver tissue lysates Lane 6: Rat Kidney tissue lysates Lane 7: Human HUVEC cell lysates Lane 8: Human K562 cell lysates Primary: Anti-PHLP (SL5812R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 40 kDa Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHLP) Polyclonal Antibody, Unconjugated (SL5812R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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