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Product Name OSGIN1 Chinese Name 氧化应激诱导生长抑制因子1抗体 Alias BDGI; Bone marrow stromal cell derived growth inhibitor; huOKL38; OKL 38; OKL38; OSGIN 1; Ovary kidney and liver protein 38; Oxidative stress induced growth inhibitor 1; Pregnancy induced growth inhibitor; Pregnancy induced growth inhibitor OKL38; OSGI1_HUMAN. Research Area Tumour Cell biology immunology Chromatin and nuclear signals Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human OSGIN1: 351-450/560 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail OSGIN1 (Oxidative stress-induced growth inhibitor 1) regulates the differentiation and proliferation of normal cells through the regulation of cell death.
Function:
Regulates the differentiation and proliferation of normal cells through the regulation of cell death.
Tissue Specificity:
Ubiquitous. Highest expression in the ovary, testis, kidney, and liver.
Similarity:
Belongs to the OKL38 family.
SWISS:
Q9UJX0
Gene ID:
29948
Database links:Entrez Gene: 29948 Human
Entrez Gene: 71839 Mouse
Omim: 607975 Human
SwissProt: Q9UJX0 Human
Product Picture Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-OSGIN1 (SL5723R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 61 kD
Observed band size: 55 kD
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-OSGIN1 (SL5723R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-OSGIN1 (SL5723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSGIN1) Polyclonal Antibody, Unconjugated (SL5723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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