Product Name	phospho-TERT (Ser1125)
Chinese Name	磷酸化端粒酶逆转录酶抗体
Alias	p-TERT(Ser1125); TERT(phospho Ser1125); TERT(phospho S1125); EST2; hEST2; TCS1; Telomerase associated protein 2; Telomerase Catalytic Subunit; Telomere Reverse Transcriptase; TERT; TP2; TRT.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=0.2μg/Test ICC=1:100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	124kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human TERT around the phosphorylation site of Ser1125: LP(p-S)DF
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that TERT cooperates with HPV E7 in cell immortalization. Ever since the discovery that telomeres are short in cancer cells and telomerase is activated in immortal cells, telomerase has been associated with oncogenes. During the past year, major advances have been made in understanding the link between telomerase expression and cell immortality. Studies of yeast telomeres have revealed an unexpected role for the non-homologous end-joining machinery in telomere maintenance and have provided the first definitive evidence that telomeres play a critical role in meiosis. Identification of new telomere proteins has led to a better understanding of vertebrate telomere structure and function. SWISS: O14746 Gene ID: 7015 Database links: Entrez Gene: 7015 Human Entrez Gene: 21752 Mouse Entrez Gene: 301965 Rat SwissProt: O14746 Human SwissProt: O70372 Mouse SwissProt: Q673L6 Rat     端粒酶逆转录酶hTERT是构成端粒酶的组分之一,是端粒酶活性的必需和限速成分,其水平决定细胞端粒酶的活性.抑制hTERT可降低端粒酶的活性,从而抑制瘤细胞生长.目前对hTERT的研究已成为端粒酶研究的热点问题,已发现TERT蛋白表达在Tumour诊断中有重要意义,并制备了hTERT抗体及应用核酶技术等来抑制hTERT蛋白的表达,抑制端粒酶活性,从而抑制Tumour的生长.     端粒反转录酶又称端粒酶催化亚单位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associated protein 2；TP2；Telomerase reverse transcriptase；telomerase catalytic subunit）是细胞永生化及恶性Tumour发生过程中的端粒酶活化的主要限速步骤。hTRT基因表达可以反映端粒酶活性，与端粒酶活性具有平行关系.
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti- phospho-TERT (Ser1125) (SL5605R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-TERT (Ser1125)) polyclonal Antibody, Unconjugated (SL5605R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (phospho-TERT (Ser1125)) polyclonal Antibody, Unconjugated (SL5605R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-phospho-TERT(Ser1125) antibody (SL5605R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. Blank control:Hela. Primary Antibody (green line): Rabbit Anti-phospho-TERT (Ser1125) antibody (SL5605R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-TERT (Ser1125) antibody (SL5605R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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