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Product Name phospho-PI 3 Kinase p110 delta (Tyr524) Chinese Name 磷酸化磷脂酰肌醇激酶催化亚单位D抗体 Alias PI 3 Kinase p110 delta (phospho Tyr524); PIK3CD(phospho Y524); PI3 Kinase p110 delta; p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphoinositide 3 kinase B; Phosphoinositide 3 kinase C; Phosphoinositide 3 kinase catalytic delta polypeptide; PI3 kinase p110 subunit delta; PI3K; PIK3CD; PK3CD; PtdIns 3 kinase p110; PI 3 Kinase p110 delta. literatures Specific References (3) | SL5587R has been referenced in 3 publications.[IF=5.561] Minrui Wu. et al. The Effect and Mechanism of Corilagin from Euryale Ferox Salisb Shell on LPS-Induced Inflammation in Raw264.7 Cells. FOODS. 2023 Jan;12(5):979 WB ; Mouse.[IF=2.795] Ying Feng. et al. LncRNA MALAT1 inhibits apoptosis of endometrial stromal cells through miR-126-5p-CREB1 axis by activating PI3K-AKT pathway. Mol Cell Biochem. 2020 Dec;475(1):185-194 WB ; Human.[IF=2.682] Deng Yang-lin. et al. Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway. J Healthc Eng. 2021;2021:3506559 WB ; Rat,Human.Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 115kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human PIK3CD around the phosphorylation site of Tyr524: EL(p-Y)EH Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p110. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K. Four isoforms of p110 has been found; the alpha, beta, gamma, and the delta subunit. The delta isoform is predominantly expressed in leukocytes and has been shown to interact with p85 and GTP-bound Ras via its SH2/SH3 domain.
Subunit:
Heterodimer of a catalytic subunit PIK3CD and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with ERAS.
Tissue Specificity:
Expressed predominantly in leukocytes.
Similarity:
Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.
SWISS:
O00329
Gene ID:
5293
Database links:Entrez Gene: 5293 Human
Omim: 602839 Human
SwissProt: O00329 Human
Unigene: 518451 Human
Product Picture Sample:
Lane 1: Rat Thymus Lysates
Lane 2: Human HT-1080 cell Lysates
Primary: Anti-phospho-PI 3 Kinase p110 delta (Tyr524)(SL5587R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kDa
Observed band size: 115 kDa
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI 3 Kinase p110 delta (Tyr524) Polyclonal Antibody, Unconjugated (SL5587R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody (SL5587R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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