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Product Name phospho-PRKCQ (Ser676) Chinese Name 磷酸化蛋白激酶C theta抗体 Alias PKC theta (phospho S676); p-PKC theta (phospho S676); PRKCQ(phospho S676); PKCθ(Phospho-Ser676); PKC theta(phospho S676); PKC 0; PRKCQ; PKC0; Prkcq; PRKCT; Protein kinase C theta; Protein kinase C theta type; Protein Kinase Ctheta; KPCT_HUMAN; nPKC theta; nPKC-theta; nPKCtheta. Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 82kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human PRKCQ around the phosphorylation site of Ser676: RL(p-S)FA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Function:
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue Specificity:
Skeletal muscle, megakaryoblastic cells and platelets.
Post-translational modifications:
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
Similarity:
Belongs to the protein kinase superfamily.
AGC Ser/Thr protein kinase family.
PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
SWISS:
Q04759
Gene ID:
5588
Database links:Entrez Gene: 5588 Human
Entrez Gene: 18761 Mouse
Omim: 600448 Human
SwissProt: Q04759 Human
SwissProt: Q02111 Mouse
Unigene: 498570 Human
Unigene: 329993 Mouse
Unigene: 225125 Rat
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