Product Name	phospho-PI3KCA (Tyr317)
Chinese Name	磷酸化磷脂酰肌醇激酶催化亚单位A抗体
Alias	PIK3CA(phospho Y317); PI3 kinase p110 subunit alpha; caPI3K; MGC142161; MGC142163; p110 alpha; Phosphatidylinositol 3 kinase catalytic alpha polypeptide; Phosphatidylinositol 3 kinase catalytic 110 KD alpha; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform; Phosphoinositide 3 kinase catalytic alpha polypeptide; PI3 kinase p110 subunit alpha; PI3K; PI3KC A; PIK3C A; PIK3CA; PK3CA; PtdIns 3 kinase p110; PI3-kinase P110α; PI3-kinase p110 subunit alpha; PI 3 Kinase catalytic subunit alpha; PK3CA_HUMAN; PI3 kinase P110α.
literatures	Specific References  (28)     |     SL5570R has been referenced in 28 publications. [IF=11.161] Chi, Ming. et al. TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer. J EXP CLIN CANC RES. 2022 Dec;41(1):1-20  WB ;  Human.   PubMed:35581606 [IF=8.101] Zhao Yin. et al. Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma. FREE RADICAL BIO MED. 2023 Jul;203:86  WB ;  Human,Mouse.   PubMed:37044150 [IF=7.701] Liu, Zhu. et al. Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica. GASTRIC CANCER. 2022 Nov;:1-17  WB ;  Human.   PubMed:36450891 [IF=6.575] Xin Liu. et al. Overexpression of YEATS2 Remodels the Extracellular Matrix to Promote Hepatocellular Carcinoma Progression via the PI3K/AKT Pathway. CANCERS. 2023 Jan;15(6):1850  WB ;  Human.   PubMed:36980736 [IF=6.376] Xu, Qinqin. et al. Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice. MOL MED. 2022 Dec;28(1):1-14  WB ;  Mouse.   PubMed:36463128 [IF=6.117] Minghui Zhang. et al. Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth. J NUTR BIOCHEM. 2023 Feb;:109277  WB ;  Mouse.   PubMed:36739096 [IF=5.923] Mingfa Ling. et al. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway. Int J Mol Sci. 2021 Jan;22(24):13352  WB ;  Mouse.   PubMed:34948148 [IF=5.89] Shilin Zhang. et al. CD73-Positive Small Extracellular Vesicles Derived From Umbilical Cord Mesenchymal Stem Cells Promote the Proliferation and Migration of Pediatric Urethral Smooth Muscle Cells Through Adenosine Pathway. FRONT BIOENG BIOTECH. 2022; 10: 895998  WB ;  Human.   PubMed:35573239 [IF=5.195] Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476  WB ;  Mouse.   PubMed:35724747 [IF=5.131] Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the α2β1/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826  WB ;  Rat.   PubMed:36684388 [IF=4.81] Yin Qin. et al. Nobiletin attenuates monocrotaline-induced pulmonary arterial hypertension through PI3K/Akt/STAT3 pathway. J PHARM PHARMACOL. 2023 May;:  WB ;  Rat.   PubMed:37158759 [IF=4.546] Yu Fei. et al. Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. CHIN MED-UK. 2022 Dec;17(1):1-14  WB ;  Human.   PubMed:36175965 [IF=4.473] Xin Jin. et al. GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells. CELL BIOL INT. 2022 Aug;:  WB ;  Bovine.   PubMed:35979663 [IF=4.36] Kun Shi. et al. Epimedii Folium and Curculiginis Rhizoma ameliorate lipopolysaccharides-induced cognitive impairment by regulating the TREM2 signaling pathway. J Ethnopharmacol. 2022 Feb;284:114766  WB ;  Mouse.   PubMed:34688798 [IF=4.221] Jingtao Zhang. et al. Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway. Journal of Traditional and Complementary Medicine. 2023 Mar;:  WB ;  Mouse.   PubMed:10.1016/j.jtcme.2023.03.008 [IF=4.081] Fan Ling. et al. Antagonizing Effects of Chromium Against Iron-Decreased Glucose Uptake by Regulating ROS-Mediated PI3K/Akt/GLUT4 Signaling Pathway in C2C12. BIOL TRACE ELEM RES. 2023 May;:1-12  WB ;  Mouse.   PubMed:37156991 [IF=3.813] Wang P et al. MiR-29 regulates the function of Goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways. J Steroid Biochem Mol Biol. 2020 Sep;202:105722.  WB ;  Goat.   PubMed:32565247 [IF=3.571] Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936.  WB ;  Mouse.   PubMed:30678459 [IF=3.571] Li X et al. Lysine enhances the stimulation of fatty acids on milk fat synthesis via the GPRC6A-PI3K-FABP5 signaling in bovine mammary epithelial cells. J Agric Food Chem. 2019 Jun 26;67(25):7005-7015.  WB ;  Cow.   PubMed:31174423 [IF=3.412] Qi H et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells.(2018) J Agric Food Chem.66(42):11027-11033.  WB ;  dairy cow.   PubMed:30274521 [IF=3.263] Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190.  WB ;  dairy cow.   PubMed:32464193 [IF=2.895] Wang K et al. Neat1-miRNA204-5p-PI3K-AKT axis as a potential mechanism for Photodynamic Therapy treated Colitis in Mice. (2018) Photodiagnosis Photodyn Ther. Oct 29.  WB ;  Mouse.   PubMed:30385297 [IF=2.813] Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13  WB ;  Rat.   PubMed:36604630 [IF=2.718] Wen Li. et al. Lysine stimulates the development of the murine mammary gland at puberty via PI3K/AKT/mTOR signalling axis. J ANIM PHYSIOL AN N. 2022 Aug;:  WB ;  Mouse.   PubMed:35923149 [IF=2.63] Luo Yan. et al. Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis. EVID-BASED COMPL ALT. 2022;2022:4194827  WB ;  Rat.   PubMed:10.1155/2022/4194827 [IF=2.563] Jiang S et al. Hesperetin as an adjuvant augments protective antitumor immunity responses in B16F10 melanoma by stimulating cytotoxic CD8+ T cells. Scandinavian Journal of Immunology, 2020: e12867.  WB ;  Mouse.   PubMed:doi:10.1111/sji.12867 [IF=2.13] Yusong Ge. et al. L-arginine stimulates the proliferation of mouse mammary epithelial cells and the development of mammary gland in pubertal mice by activating the GPRC6A/PI3K/AKT/mTOR signalling pathway. J ANIM PHYSIOL AN N. 2022 May 26  WB ;  Mouse.   PubMed:35616019 [IF=2.05] Fan QC et al. Integrin-α5 promoted the progression of Oral Squamous Cell Carcinoma and modulated PI3K/AKT Signaling Pathway.Arch Oral Biol. 2019 May;101:85-91.  WB ;  Human.   PubMed:30909080
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	117kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317: TP(p-Y)MN
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach. SWISS: P42336 Gene ID: 5290 Database links: Entrez Gene: 5290 Human Omim: 171834 Human SwissProt: P42336 Human Unigene: 553498 Human Unigene: 715194 Human Unigene: 85701 Human
Product Picture	Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 3: Jurkat (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Lane 6: Siha (Human) Cell Lysate at 30 ug Primary: Anti-phospho-PI3KCA (Tyr317) (SL5570R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/120 kD Observed band size: 110 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3KCA (Tyr317)) Polyclonal Antibody, Unconjugated (SL5570R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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