Product Name	phospho-PTK2 (Tyr576+Tyr577)
Chinese Name	磷酸化粘着斑激酶抗体
Alias	PTK2 (phospho Y576/Y577); FAK (phospho Y576 + Y577); FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	119kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr576/577: ST(p-Y)(p-Y)KA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions. Subcellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Tissue Specificity: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. SWISS: Q05397 Gene ID: 5747 Database links: Entrez Gene: 5747 Human Entrez Gene: 14083 Mouse Entrez Gene: 25614 Rat Omim: 600758 Human SwissProt: Q05397 Human SwissProt: P34152 Mouse SwissProt: O35346 Rat Unigene: 395482 Human Unigene: 254494 Mouse Unigene: 2809 Rat     FAK是整合蛋白介导的Signal transduction中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化,FAK本身是胱冬肽酶(caspase)的底物。作为信号分子的FAK参与抑制Apoptosis并直接参与细胞多种功能的调节。 1.FAK 局部粘着斑激酶，是一种酪氨酸激酶;Tumour细胞的侵袭性生长是一个多步骤的复杂过程，有多种生物化学因子参与其中,局部粘着斑激酶（focal adhesion kinase, FAK）介导的Signal transduction系统就是其中最为重要的细胞Signal transduction途径之一。Tumour细胞必须黏附于Extracellular matrix，通过促进依赖于PTK激酶活性的Extracellular matrixSignal transduction，进而影响细胞的黏附、运动与迁移。 2.粘着斑激酶(focal adhesion kinase,FAK)是整合蛋白介导的Signal transduction中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化；为信号分子的FAK,还与细胞内其他Signal transduction通路存在串话(crosstalk),直接参与了细胞多种功能的调节。 3.尽管FAK的确切功能尚不清楚,但若干实验均提示FAK可能有两个作用,一是在细胞铺展和移动时,FAK参与粘着斑形成和调节；二是FAK参与Signal transduction过程,以告知The nucleus其细胞已锚定了。近年有关FAK在Apoptosis中的作用也业已肯定。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PTK2 (Tyr576+Tyr577)) Polyclonal Antibody, Unconjugated (SL5541R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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