Product Name	HMGB1
Chinese Name	高迁移率族蛋白B1抗体
Alias	High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
Research Area	Tumour  Cell biology  immunology  transcriptional regulatory factor  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:50-100 IF=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human HMGB1: 100-200/215
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. SWISS: P09429 Gene ID: 3146 Database links: Entrez Gene: 282691 Cow Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Entrez Gene: 25459 Rat Omim: 163905 Human SwissProt: P10103 Cow SwissProt: P09429 Human 近来的研究表明称之为高迁移率族蛋白B-1（HMG-B1）的核内结构蛋白在核外表达时是一种有效的早期炎症介质。 高迁移性Ｂ１组蛋白（ＨＭＧＢ１）： 是一种核Binding protein，在ＤＮＡ重组、修复、复制和基因转录中起作用。ＨＭＧＢ１也是巨噬细胞分泌的一种介质。 此外，高迁移性Ｂ１组蛋白亦被受刺激的巨噬细胞或单核细胞主动分泌。在这一主动分泌过程中，HMGB1首先经乙酰化并由核内转移至溶酶体内，继而在ATP和溶血磷脂胆碱两种分泌信号指导下转移至细胞外。由坏死细胞被动释放的HMGB1和炎症细胞主动分泌的HMGB1存在分子上的差异。胞外的HMGB1可作为cell factor参与信号传导，因为它既可识别Toll 样受体（TLR）家族的一些成员，又能与识别晚期糖基化终末产物受体（RAGE）相作用。 HMGB1能启动炎症反应，包括产生多种cell factor、对某些Stem cells产生趋化作用、诱导血管粘附分子、削弱肠epithelial cells的功能等
Product Picture	Sample: Lane 1: Human Hela Cell (Control) Lysate Lane 2: HMGB1 knockout (KO) HeLa Cell Lysate Primary: Anti-HMGB1 (SL55098R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 30 kD Sample: Lane 1: Lung (Mouse) TissueLysate at 40 ug Lane 2: Liver (Mouse) Tissue Lysate at 40 ug Lane 3: Testis (Rat) Tissue Lysate at 40 ug Lane 4: Cerebrum (Rat) Tissue Lysate at 40 ug Lane 5: Jurkat (Human) Cell Lysate at 30 ug Lane 6: Hela (Human) Cell Lysate at 30 ug Lane 7: HepG2 (Human) Cell Lysate at 30 ug Lane 8: Molt-4 (Human) Cell Lysate at 30 ug Primary: Anti-HMGB1 (SL55098R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (SL55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (SL55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (SL55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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