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Product Name phospho-MyoD1 (Ser200) Chinese Name 磷酸化肌原调节蛋白抗体 Alias MyoD1 (phospho Ser200); MyoD1 (phospho S200); p-MyoD1 (phospho S200); MYOD1(phospho S200);Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN. literatures Specific References (1) | SL5499R has been referenced in 1 publications.[IF=4.938] Du J et al. The regulation of skeletal muscle fiber-type composition by betaine is associated with NFATc1/MyoD.J Mol Med (Berl). 2018 Jun 6. WB ; Mouse.Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human MyoD1 around the phosphorylation site of Ser200: DA(p-S)SP Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Function:
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5 (By similarity).
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.
SWISS:
P15172
Gene ID:
4654
Database links:Entrez Gene: 374048 Chicken
Entrez Gene: 4654 Human
Entrez Gene: 17927 Mouse
Entrez Gene: 443405 Sheep
Omim: 159970 Human
SwissProt: P16075 Chicken
SwissProt: P15172 Human
SwissProt: P10085 Mouse
SwissProt: P29331 Sheep
Unigene: 181768 Human
Unigene: 1526 Mouse
Unigene: 9493 Rat
Product Picture Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Tongue tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Primary: Anti- phospho-MyoD1 (Ser200) (SL5499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-MyoD1 (Ser200) antibody (SL5499R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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