Product Name	phospho-MAP4K1 (Ser171)
Chinese Name	磷酸化造血祖细胞激酶抗体
Alias	MAP4K1(phospho S171); Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen Activated Protein Kinase Kinase Kinase Kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1.
Product Type	Phosphorylated anti
Research Area	immunology  Signal transduction  Kinases and Phosphatases  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Horse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	91kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human MAP4K1 around the phosphorylation site of Ser171: RL(p-S)FI
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Function: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Subunit: Interacts with MAP3K1. Tissue Specificity: Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. SWISS: Q92918 Gene ID: 11184 Database links: Entrez Gene: 11184 Human Entrez Gene: 26411 Mouse Entrez Gene: 100516374 Pig Entrez Gene: 292763 Rat Omim: 601983 Human SwissProt: Q92918 Human SwissProt: P70218 Mouse Unigene: 95424 Human Unigene: 148278 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-MAP4K1(Ser171)) Polyclonal Antibody, Unconjugated (bs5494R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MAP4K1 Antibody(SL5494R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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