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Product Name phospho-ERK5 (Ser496) Chinese Name 磷酸化细胞外信号调节激酶5抗体 Alias ERK5 (phospho S496); Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK-5; ERK4; Extracellular Signal Regulated Kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen Activated Protein Kinase 7; Mitogen-activated protein kinase 7; MK07_HUMAN; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE, MITOGEN-ACTIVATED, 7. literatures Specific References (3) | SL5484R has been referenced in 3 publications.[IF=7.36] Ye F et al. Whole exome and target sequencing identifies MAP2K5 as novel susceptibility gene for familial non-medullary thyroid carcinoma. Int J Cancer. 2019 Mar 15;144(6):1321-1330. ICF&WB ; Human.[IF=5.378] Zhuang et al. CDK5 functions as a tumor promoter in human colorectal cancer via modulating the ERK5-AP-1 axis. (2016) Cell.Death.Di. 7:e2415 IHC-P ; Mouse.[IF=5.162] Lei Zhao. et al. Proteomic analysis reveals the molecular mechanism of Hippophae rhamnoides polysaccharide intervention in LPS-induced inflammation of IPEC-J2 cells in piglets. Int J Biol Macromol. 2020 Dec;164:3294 WB ; Pig.Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 90kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Ser496: GP(p-S)AP Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function:
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression.
Subunit:
Interacts with MAP2K5. Forms oligomers (By similarity). Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D (By similarity). Interacts with SGK1.
Subcellular Location:
Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
Tissue Specificity:
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
Post-translational modifications:
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
Q13164
Gene ID:
5598
Database links:Entrez Gene: 5598 Human
Entrez Gene: 23939 Mouse
Omim: 602521 Human
SwissProt: Q13164 Human
SwissProt: Q9WVS8 Mouse
Unigene: 150136 Human
Unigene: 38172 Mouse
Unigene: 144629 Rat
Product Picture Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: Hcclm3 (Human) Cell Lysate at 30 ug
Primary:
Anti- phospho-ERK5 (Ser496) (SL5484R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ERK5 (Ser496)) Polyclonal Antibody, Unconjugated (SL5484R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human myometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ERK5(Ser496) Polyclonal Antibody, Unconjugated(SL5484R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ERK5(Ser496) Polyclonal Antibody, Unconjugated(SL5484R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ERK5 (Ser496)) polyclonal Antibody, Unconjugated (SL5484R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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