Rabbit Anti-phospho-MBP (Tyr203)antibody
MBP(phospho Tyr203); MBP(phospho Y203); Myelin basic protien; GDB; Golli MBP; Hemopoietic MBP; HMBPR; HUGO; MBP; MGC99675; MLD; Myelin A1 Protein; Myelin Deficient; Myelin Membrane Encephalitogenic Protein; SHI; Shiverer; SP.
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Product Name phospho-MBP (Tyr203) Chinese Name 磷酸化髓鞘碱性蛋白抗体 Alias MBP(phospho Tyr203); MBP(phospho Y203); Myelin basic protien; GDB; Golli MBP; Hemopoietic MBP; HMBPR; HUGO; MBP; MGC99675; MLD; Myelin A1 Protein; Myelin Deficient; Myelin Membrane Encephalitogenic Protein; SHI; Shiverer; SP. Product Type Phosphorylated anti Research Area immunology Neurobiology Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, ) Applications ELISA=1:5000-10000 Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human MBP around the phosphorylation site of Tyr203: AH(p-Y)GS Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Oligodendrocyte Marker
The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
Function:
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Subunit:
Homodimer. Isoform 3 exists as a homodimer.
Subcellular Location:
Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
Tissue Specificity:
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Post-translational modifications:
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.
Similarity:
Belongs to the myelin basic protein family.
SWISS:
P02686
Gene ID:
4155
Database links:Entrez Gene: 4155 Human
Entrez Gene: 17196 Mouse
Omim: 159430 Human
SwissProt: P02686 Human
SwissProt: P04370 Mouse
SwissProt: P25274 Rabbit
Unigene: 551713 Human
Unigene: 63285 Rat
少突胶质细胞Maker。
主要用于脊髓脱髓鞘病-脊髓多发硬化症的研究。 MBP髓鞘碱性蛋白和髓鞘相伴glycoprotein是多发性硬化的自身免疫攻击的靶。Product Picture 
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-phospho-MBP (Tyr203) antibody (SL5475R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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