Product Name	phospho-MEK5 (Ser129)
Chinese Name	磷酸化丝裂原活化蛋白激酶激酶5抗体
Alias	MAP2K5(phospho S129); Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5_HUMAN; PRKMK5; Protein kinase, mitogen-activated, kinase 5; SAPKK5; SKK5.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human MEK5 around the phosphorylation site of Ser129: GP(p-S)QH
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle. Function: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis. Subunit: Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ. Tissue Specificity: Expressed in many adult tissues. Abundant in heart and skeletal muscle. Post-translational modifications: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 OPR domain. Contains 1 protein kinase domain. SWISS: Q13163 Gene ID: 5607 Database links: Entrez Gene: 5607 Human Entrez Gene: 23938 Mouse Entrez Gene: 29568 Rat Omim: 602520 Human SwissProt: Q13163 Human SwissProt: Q9WVS7 Mouse SwissProt: Q62862 Rat Unigene: 114198 Human Unigene: 325746 Mouse Unigene: 11054 Rat
Product Picture	Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-phospho-MEK5 (Ser129) (SL5431R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MEK5 (Ser129)) Polyclonal Antibody, Unconjugated (SL5431R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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