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Product Name Seladin 1 Chinese Name 24脱氢胆固醇还原酶抗体 Alias Seladin1; Seladin-1; DHCR24; 24 dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Desmosterol to cholesterol enzyme; Diminuto dwarf1 homolog; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1. literatures Specific References (1) | SL5390R has been referenced in 1 publications.[IF=1.227] Zhang XD et al. MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene. Asian-Australas J Anim Sci. 2019,July 1. WB ; Pig.Research Area Tumour immunology Neurobiology Signal transduction Apoptosis transcriptional regulatory factor Alzheimer's Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Seladin 1: 101-200/516 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Seladin 1 is highly expressed in brain, adrenal tissues and adrenal tumours, where this enzyme catalyzes the reduction of the delta-24 double bond of sterol intermediates such as desmosterol, during cholesterol biosynthesis. In addition, Seladin 1 has an anti-apoptotic function by reducing caspase 3 activity during oxidative stress-induced apoptosis and protecting cells against amyloid-beta peptide-induced apoptosis. Several studies have identified a reduction in expression levels of this enzyme in brain regions affected by Alzheimer's disease (hippocampus and the subventricular zone).
Subunit:
Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.
Tissue Specificity:
Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.
Similarity:
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
Contains 1 FAD-binding PCMH-type domain.
SWISS:
Q15392
Gene ID:
1718
Database links:Entrez Gene: 1718 Human
Entrez Gene: 74754 Mouse
Omim: 606418 Human
SwissProt: Q15392 Human
SwissProt: Q8VCH6 Mouse
Unigene: 498727 Human
Unigene: 133370 Mouse
Unigene: 225146 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Seladin 1) Polyclonal Antibody, Unconjugated (SL5390R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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