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Product Name PLCZ1 Chinese Name 磷酸肌醇磷脂酶PLCZ1抗体 Alias 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1; Phosphoinositide phospholipase C zeta 1; Phospholipase C zeta 1; PI phospholipase C zeta 1; PLC zeta 1; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; MGC149685 antibody NYD SP27 antibody. literatures Specific References (3) | SL5378R has been referenced in 3 publications.[IF=6.684] Meng Wang. et al. A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure. Front Cell Dev Biol. 2021; 9: 672081 IF ; Human.[IF=3.322] Xiaopei Zhou. et al. Actl7a deficiency in mice leads to male infertility and fertilization failure. BIOCHEM BIOPH RES CO. 2022 Oct;623:154 IF ; Mouse.[IF=2.98] Zhou, Xiaopei. et al. A novel homozygous mutation in ACTL7A leads to male infertility. MOL GENET GENOMICS. 2022 Dec;:1-8 IF ; Human.Research Area immunology transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 70kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PLCZ1: 351-450/608 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, PLCZ1 hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. It triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. PLCZ1 is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. It may exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Tissue Specificity:
Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
SWISS:
Q86YW0
Gene ID:
89869
Database links:Entrez Gene: 89869 Human
Entrez Gene: 114875 Mouse
Omim: 608075 Human
SwissProt: Q86YW0 Human
SwissProt: Q8K4D7 Mouse
Unigene: 97542 Human
Unigene: 50808 Mouse
Unigene: 123184 Rat
Product Picture Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLCZ1 Polyclonal Antibody, Unconjugated(SL5378R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (SL5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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