Product Name	phospho-HSF1 (Ser307)
Chinese Name	磷酸化热休克因子1抗体
Alias	HSF1 (phospho S307); p-HSF1 (phospho S307); HSF1 (Phospho-Ser307); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1; HSF1_HUMAN.
literatures	Specific References  (1)     |     SL5364R has been referenced in 1 publications. [IF=9.429] Hao, Jie. et al. An IFI6-based hydrogel promotes the healing of radiation-induced skin injury through regulation of the HSF1 activity. J NANOBIOTECHNOL. 2022 Dec;20(1):1-14  WB ;  Mouse.   PubMed:35717249
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser307: PQ(p-S)PR
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]. Function: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher ukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Subunit: Monomer. Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2. Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. Post-translational modifications: Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Sumoylated by SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. Similarity: Belongs to the HSF family. SWISS: Q00613 Gene ID: 3297 Database links: Entrez Gene: 3297 Human Omim: 140580 Human SwissProt: Q00613 Human Unigene: 530227 Human
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Pancreas tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Mouse Lung tissue lysates Lane 5: Mouse Large intestine tissue lysates Lane 6: Mouse NIH/3T3 cell lysates Primary: Anti-phospho-HSF1 (Ser307) (SL5364R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 80 kD

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