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Product Name phospho-Separase (Ser1073) Chinese Name 磷酸化外纺锤体极样蛋白1抗体 Alias Caspase like protein ESPL1; ESP 1; ESP1; ESP-1; ESPL 1; ESPL1; ESPL-1; Extra spindle poles like 1; Extra spindle poles like 1 protein; Separin; Similar to fission yeast cut1and gene; SSE; Separase; ESPL1_HUMAN. Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction Transporter Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 198kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073: LD(p-S)VK Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers.
Function:
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Subunit:
Interacts with PTTG1. Interacts with RAD21.
Subcellular Location:
Cytoplasmic and Nuclear
Post-translational modifications:
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Similarity:
Belongs to the peptidase C50 family.
SWISS:
Q14674
Gene ID:
9700
Database links:Entrez Gene: 9700 Human
Entrez Gene: 105988 Mouse
Omim: 604143 Human
SwissProt: Q14674 Human
SwissProt: P60330 Mouse
Unigene: 153479 Human
Unigene: 288324 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-Separase (Ser1073)) Polyclonal Antibody, Unconjugated (SL5304R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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