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Product Name phospho-CDC27 (Thr244) Chinese Name 磷酸化后期促进复合蛋白3抗体 Alias ANAPC3; Anaphase Promoting Complex 3; Anaphase promoting complex protein 3; Anaphase Promoting Complex Subunit 3; Anaphase-promoting complex subunit 3; APC 3; APC3; APC-3; Cdc 27; Cdc27; Cdc-27; CDC27 homolog; CDC27_HUMAN; CDC27Hs; Cell division cycle 27; Cell division cycle protein 27 homolog; H NUC; H-NUC; HNUC; NUC2 homolog. Product Type Phosphorylated anti Research Area Cell biology immunology Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Dog, Pig, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 92kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244: PD(p-T)VP Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [PATHWAY] Protein modification; protein ubiquitination.
Subunit:
The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI (By similarity). Interacts with MCPH1.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
Similarity:
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
SWISS:
P30260
Gene ID:
996
Database links:Entrez Gene: 996 Human
Entrez Gene: 217232 Mouse
Omim: 116946 Human
SwissProt: P30260 Human
SwissProt: A2A6Q5 Mouse
Unigene: 463295 Human
Unigene: 89845 Mouse
Unigene: 198335 Rat
Product Picture Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GSK-3 Beta (Ser21) Polyclonal Antibody, Unconjugated(SL5368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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