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Product Name phospho-CTNNBIP1 (Thr43+Ser50) Chinese Name 磷酸化β链接素相互作用蛋白1抗体 Alias Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN. Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 9kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human CTNNBIP1 around the phosphorylation site of Thr43/Ser50: LR(p-T)YAGVVN(p-S)QL Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway.
Function:
Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.
Subunit:
Binds CTNNB1.
Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity).
Similarity:
Belongs to the CTNNBIP1 family.
SWISS:
Q9NSA3
Gene ID:
56998
Database links:Entrez Gene: 56998 Human
Entrez Gene: 67087 Mouse
Omim: 607758 Human
SwissProt: Q9NSA3 Human
SwissProt: Q9JJN6 Mouse
Unigene: 463759 Human
Unigene: 299735 Mouse
Unigene: 162132 Rat
Product Picture Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-CTNNBIP1(Thr43+Ser50) Polyclonal Antibody, Unconjugated(SL5266R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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