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Product Name phospho-ATG4A (Ser100) Chinese Name 磷酸化自噬相关蛋白4A抗体 Alias ATG4A (phospho Ser100); ATG4A (phospho S100); AI627006; Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A; MGC107179. Product Type Phosphorylated anti Research Area Tumour Cardiovascular Cell biology immunology Signal transduction Apoptosis Mitochondrion Extracellular matrix Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization cytoplasmic Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human ATG4A around the phosphorylation site of Ser100: DW(p-S)WE Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Function:
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Subcellular Location:
Cytoplasm (Probable).
Tissue Specificity:
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Similarity:
Belongs to the peptidase C54 family.
SWISS:
Q8WYN0
Gene ID:
115201
Database links:Entrez Gene: 115201 Human
Entrez Gene: 666468 Mouse
Omim: 300663 Human
SwissProt: Q8WYN0 Human
SwissProt: Q8C9S8 Mouse
Unigene: 8763 Human
Unigene: 102230 Mouse
Unigene: 393320 Mouse
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